The Next Generation in Membrane Protein Structure Determination
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Membrane protein structure determination - the next generation.
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Membrane protein structure determination — The next generation
Crystal structure analysis of a voltage-gated sodium channel. In recent years, several research teams from Stockholm University have made progress in their efforts to determine the structure of human membrane proteins using different methods.
One of the greatest challenges is to combine better methods for protein expression, purification, and crystallisation using modern computer-based methods, such as bioinformatics and simulations. The biggest challenge when it comes to determining crystal structures is that it is far from certain that the researchers will manage to obtain crystals, and even if they do, there is a risk that some of the membrane proteins will be too unstable for the crystals to be arranged properly.
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To avoid these problems, the researchers can also use cryo-EM, where they rapidly freeze a small membrane sample and use an electron microscope to take pictures of individual proteins. Traditionally, this method has only resulted in low-resolution structures, but a new cryo-EM facility at Stockholm University will enable researchers to use a new generation of tools to detect electrons directly with much higher resolution.
By combining thousands of images with computer models, the researchers will be able to gradually obtain information on what the structures look like, what states they appear in, and how membrane proteins interact with each other. A new research grant worth SEK 29 million from the Knut and Alice Wallenberg Foundation to David Drew and Stockholm University will make it possible to take the research on membrane-bound proteins a step further.
Research on membrane proteins and protein structure determination at Stockholm University is strengthened further by several large grants to Professor Gunnar von Heijne at the Department of Biochemistry and Biophysics.
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The four research teams mentioned above, and a world-leading research team from Munich, are among those who will conduct their research in this laboratory. Stockholm University. To submenu. Concentrated effort to gain new knowledge about membrane-bound proteins.